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Enzyme linked immunosorbent assay ELISA
2022-08-17

Enzyme-linked immunosorbent assay (ELISA) is a rapid and simple test widely used for the detection of humoral immune responses following infection/vaccination. However, commercially available ILTV ELISA use the whole virus as the detecting antigen and therefore may not be sensitive for the detection
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Given that we planned to isolate the GluN GluN A
2022-08-17

Given that we planned to isolate the GluN1/GluN3A receptor by immunoaffinity chromatography using a Myc epitope tag on GluN3A, we first checked that the tag does not affect the functional properties of the receptor. To do this we co-transfected HEK-293 cells with DNA encoding WT GluN1 and either WT
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During pain signal transmission glutamate is released into t
2022-08-17

During pain signal transmission, glutamate is released into the synaptic cleft, which, in turn, causes activation of N-methyl-d-aspartate (NMDA) receptors and hence neurotransmission across the synapse occurs. The synaptic concentration and resultant activity of glutamate are controlled by excitator
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br Declaration of interest br
2022-08-17

Declaration of interest Acknowledgments This work was financially supported by the National Natural Science Foundation of China (No. 31471615), the Introduction of Talent Research Start-up Fund of Anhui Polytechnic University (No. 2017YQQ017) and the Pre-research Project of National Natural Sc
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Rifampicin that is methyl piperazinyl imino
2022-08-17

Rifampicin, that is 3-[[(4-methyl-1-piperazinyl) imino] methyl]-rifamycin (Fig. 1), is a semisynthetic antibiotic drug. It is one of the most potent and broad drug against bacterial pathogens and tuberculosis [9]. In recent years, rifampicin has also been reported to have inhibitory effects on Taq R
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L Family Leguminosae widely distributed in tropical and subt
2022-08-17

L. (Family Leguminosae) widely distributed in tropical and subtropical regions of the world. As a medicinal and edible plant, it is widely used in Asia, especially in China, Korea, Japan and India. It was first recorded in Shen Nong Ben Cao Jing (神农本草经), and was described having the effect of cleari
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For another the N terminal
2022-08-17

For another, the N-terminal sequence of native glucagon is highly conserved, and positions 8, 9, 16 and 18 are noteworthy in maintaining glucagon activity [18], [20], [21]. It also achieves a degree of selectivity from its C-terminal residues, and a significant contribution is made by the C-terminal
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Hypocotyl development is a crucial phase of early
2022-08-16

Hypocotyl development is a crucial phase of early vegetative growth that influences seedling vigor contributing to fitness and productivity of a plant system (Stinchcombe and Schmitt, 2006; Luo et al., 2017). The development of a hypocotyl is characterized by an initial slow growth phase followed by
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Serotonin hydroxytryptamine HT is a monoamine with
2022-08-16

Serotonin (5-hydroxytryptamine, 5-HT) is a monoamine with dual functions in the developing and matured brain. 5-HT regulates development of the RS-1 as a neurotrophic factor and is involved in emotion and cognition as a neurotransmitter in adulthood (Gaspar et al., 2003; Daubert and Condron, 2010;
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Tazobactam sodium salt uPAR promotes cell associated proteol
2022-08-16

uPAR promotes cell-associated proteolysis by binding to uPA which locally converts plasminogen into active plasmin [10]. Ligand-engaged uPAR also acts as a potent regulator of cell migration and matrix attachment. Being a membrane receptor, uPAR needs to interact with extracellular matrix (ECM) prot
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All of the abovementioned effects
2022-08-16

All of the abovementioned effects of PUFAs signal their involvement in a cellular phenotype switching phenomenon, which is increasingly recognized as a potential target for future pharmacotherapies of atherosclerosis, especially for individuals who do not respond to the classical lipid-lowering stra
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Enhancer of zeste homolog EZH which
2022-08-16

Enhancer of zeste homolog 2 (EZH2), which functions as a methyltransferase, is a critical subunit of polycomb repressive complex 2 (PRC2), which has been demonstrated to be involved in the development of the Nemadectin [23]. Aberrant expression of EZH2 has been found to be associated with a variety
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br Acknowledgments I thank Takeshi Sakaba for critical readi
2022-08-16

Acknowledgments I thank Takeshi Sakaba for critical reading of the manuscript and helpful comments. This work was supported by JSPS/MEXT KAKENHI Grant Numbers 17K19466, 17H03548. Introduction The oral route of drug application is the main route in pharmacology since it is easy to handle and o
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The observed tendency for higher plasma lactate
2022-08-16

The observed tendency for higher plasma lactate levels in the aerated thermal exposure suggests increased reliance on SB742457 australia metabolism to maintain function. This finding is supported by a recent study that observed an increased ṀO2 in European perch (Perca fluviatilis) exposed to a hype
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br Recent insights into EAAT structure function aspects The
2022-08-15

Recent insights into EAAT structure/function aspects The EAATs are secondary-active transporters coupling the movement of one Glu with the symport of three Na+ and one proton and the counter-transport of one K+ [3, 4]. The transporters are trimeric assemblies of protomers that function independen
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